Maturation of membrane properties of neurons in the rat deep cerebellar nuclei

Desheng Wang, Bernard G. Schreurs
2014 Developmental Neurobiology  
Patch clamp recordings of neurons in the adult rat deep cerebellar nuclei have been limited by the availability of viable brain slices. Using a new slicing technique, this study was designed to explore the maturation of membrane properties of neurons in the deep cerebellar nuclei (DCN),an area involved in rat eyeblink conditioning. Compared to whole-cell current clamp recordings in DCN in rat pups at postnatal day 16 (P16) to P21, recordings from weanling rats at P22 to P40 revealed a number of
more » ... significant changes including an increase in the amplitude of the afterhyperpolarization (AHP) -an index of membrane excitability which has been shown to be important for eyeblink conditioning -a prolonged interval between the 1 st and 2 nd evoked action potential, and an increase in AHP amplitude for hyperpolarization-induced rebound spikes. This is the first report of developmental changes in membrane properties of DCN which may be a major contributor to the ontogeny of eyeblink conditioning in the rat.
doi:10.1002/dneu.22203 pmid:24931427 pmcid:PMC4211972 fatcat:ypp7g6z2a5alxa73ce24onls7i