A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2019; you can also visit the original URL.
The file type is application/pdf
.
The effect of combination of methanol with several concentration of honey solution on sperm quality of botia Chromobotia macracanthus Bleeker 1852 after short-term storage at −80 ℃
[post]
2019
unpublished
Clown loach or botia, Chromobotia macracanthus Bleeker 1852, is a popular freshwater ornamental fish. Lack of high quality broodstock and asynchronous gonad maturation are the main problem in breeding of this species. Cryopreservation is one of the methods to overcome this problem, and cryoprotectant plays a vital role in this process. Herein, we tested two types of cryoprotectants, namely methanol as a permeating cryoprotectant and honey solution as a natural non-permeating cryoprotectant.
doi:10.21203/rs.2.17253/v1
fatcat:73lzcjpvavgexhzpz64vutkhgi