Defining amino acid pairs as structural units suggests mutation sensitivity to adjacent residues [article]

Aviv A. Rosenberg, Nitsan Yehishalom, Ailie Marx, Alex Bronstein
2022 bioRxiv   pre-print
AbstractProteins fold from chains of amino acids, forming secondary structures, α-helices and β-strands, that, at least for globular proteins, subsequently fold into a three-dimensional structure. A large-scale analysis of high-resolution protein structures suggests that amino acid pairs constitute another layer of ordered structure, more local than these conventionally defined secondary structures. We develop a cross-peptide-bond Ramachandran plot that captures the conformational preferences
more » ... the amino acid pairs and show that the effect of a particular mutation on the stability of a protein depends in a predictable manner on the adjacent amino acid context.One-Sentence SummaryLarge-scale protein backbone analysis reveals amino acid pair conformational preferences and predicts how sequence context affects mutant stability.
doi:10.1101/2022.10.23.513383 fatcat:a52ebofltbgxdalrbyedeqfeua