PHYTOCHEMICAL INVESTIGATION AND EVOLUATION OF THE BIOLOGICAL EFFECT OF CYPERUS SECONDARY METABOLITES ON FERTILITY IN MALE MICE

Wid Ibrahim Kadm
2017 World Journal of Pharmaceutical Research  
The present study was designed to detect the effect of Cyperus esculentus extract on male albino mice fertility. C. esculentus extract was prepared by maceration of 50 gm of C. esculentus tubers with 90% methanol, then chemical detection of flavonoids, alkaloids, tannins, saponins, terpenes and steroids was carried out. The use of high performance liquid chromatography technique helped in detection of querciten, rutin, kaempferol, Myricetin and Luteolin. The effect of C. esculentus methanolic
more » ... lentus methanolic extract on the sperm including sperms concentration, percentage of viable sperms, percentage of morphologically abnormal sperms and an assay of serum testosterone were studied. Twenty five mice were divided equally into five groups, including negative and positive controls while other groups were treated with different concentrations of C. esculentus extract. The extracts were administered orally for 3 weeks. The results showed a significant increase (p > 0.01) in sperms concentration after 3 weeks of treatment with the C. esculentus extract at doses 200, 400 and 600 mg/kg when compared with controls. A significant decrease (p ≤ 0.01) in dead sperm and percentage of morphologically abnormal sperms was observed after treatment with C. esculentus extract at doses (200, 400 and 600) mg/kg when compared with controls. A significant increase (p ≤ 0.01) in Serum testosterone was observed in mice treated with 200 and 400 mg/kg when compared with controls and with another group treated with C. esculentus extract at dose of 600 mg/kg.
doi:10.20959/wjpr20178-8867 fatcat:p6jitiwmnrbt3kngzj77atgw74