Beta 2-microglobulin in neutrophils: an intragranular protein

O W Bjerrum, O J Bjerrum, N Borregaard
1987 Journal of Immunology  
The subcellular localization of beta2-microglobulin (beta 2m) in human neutrophils was determined by an enzyme-linked immunosorbent assay on subcellular fractions obtained by Percoll density gradient centrifugation of neutrophils disrupted by nitrogen cavitation. The neutrophils were found to contain 160 ng beta 2m/mg protein. Approximately two-thirds co-located with the markers for specific granules and was released from intact cells during degranulation, whereas one-third of the beta 2m was
more » ... cated together with markers of the plasma membrane. This fraction was not further enriched during degranulation. These results indicate that beta 2m cannot be universally used as a plasma membrane marker as hitherto assumed, but beta 2m may serve as an indicator of neutrophil degranulation.
doi:10.4049/jimmunol.138.11.3913 fatcat:edlgovh2wbey3kshpxrshvntai