Correlation between DNA damage and GST-P in hepatocytes of juvenile rats treated with diethylnitrosamine

Izumi Ogawa, Satoshi Furukawa, Masayoshi Abe, Yoshinori Tanaka, Koji Usuda
2004 Environmental Mutagen Research  
We investigated the relationship between DNA damage detected as single strand breaks using a Comet assay and the expression of glutathione S-transferase P-form(GST-P)as a marker of the early stage of carcinogenesis. In this study, 4-week-old rats were exposed to 10 or 40 ppm diethylnitrosamine(DEN)in drinking water for 14 days, then both DNA damage and GST-P expression were evaluated in the three primary lobes[left(L) , right median(RM)and right anterior(RA) ] of the liver to clarify the
more » ... tion between two endpoints within the organ over time. Both DNA damage and GST-P expression increased almost in parallel in a dose-and time-dependent manner in all lobes, and those had a good relationship with heterogeneous response, L ≧ RM>RA. Therefore, it was confirmed that the result of the Comet assay could correspond to the histopathological changes appearing in the initiation stage in rat hepatocytes.
doi:10.3123/jems.26.75 fatcat:76z3mjp74ndqhpf4qkhppcmn34