Parametric Optimization of Extracellular Chitin Deacetylase Production by Scopulariopsis brevicaulis

Jun Cai Jin Li
2013 Journal of Biocatalysis & Biotransformation  
The culture medium, fermentation conditions and effects of different inorganic salts on producing extracellular chitin deacetylase by Scopulariopsis brevicaulis were optimized. The highest enzymatic activity of deacetylation of chitin was 36 units/mL for optimum culture medium which included 2% (w/v) 3,6-O-carboxymethylchitin, 1% sucrose, 0.2% NaNO 3 , 0.1% K 2 HPO 4 , 0.05% KCl, 0.4% peptone, 0.05% MgSO 4 , 0.05% MnSO 4 , 0.03% CoCl 2 and optimum cultural conditions included temperature, pH,
more » ... temperature, pH, agitation speed, fermenting time and volume and were 29°C, 7.0, 200 rmp min -1 , 96 hours and 80 mL in 300 mL Erlenmeyer flask, respectively. The capacity for producing chitin deacetylase was increased 76%, 70% and 68% when 0.05% (w/v) MnSO 4 , 0.03% (w/v) CoCl 2 and 0.05% (w/v) MgSO 4 were added to culture solution, respectively.
doi:10.4172/2324-9099.1000103 fatcat:mlc4ktgppbco7ccdzgumwtieim