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The culture medium, fermentation conditions and effects of different inorganic salts on producing extracellular chitin deacetylase by Scopulariopsis brevicaulis were optimized. The highest enzymatic activity of deacetylation of chitin was 36 units/mL for optimum culture medium which included 2% (w/v) 3,6-O-carboxymethylchitin, 1% sucrose, 0.2% NaNO 3 , 0.1% K 2 HPO 4 , 0.05% KCl, 0.4% peptone, 0.05% MgSO 4 , 0.05% MnSO 4 , 0.03% CoCl 2 and optimum cultural conditions included temperature, pH,doi:10.4172/2324-9099.1000103 fatcat:mlc4ktgppbco7ccdzgumwtieim