Cultural and physiological characteristics of Clostridium botulinum type G and the susceptibility of certain animals to its toxin

A S Ciccarelli, D N Whaley, L M McCroskey, D F Gimenez, V R Dowell, C L Hatheway
1977 Applied and Environmental Microbiology  
Strain 89 of Clostridium botulinum type G, isolated by Gimenez and Ciccarelli in 1969, was characterized culturally, biochemically, and toxigenically. It was motile, hemolytic, asaccharolytic, weakly proteolytic, lipase and lecithinase negative, and it produced acetic, isobutyric, butyric, and isovaleric acids in peptoneyeast extract-glucose broth. No spores were seen in smears from solid or liquid media. Very low levels of toxin were produced in regular broth cultures, but dialysis cultures
more » ... ialysis cultures yielded 30,000 mouse 50% mean lethal doses (LD50) per ml.
doi:10.1128/aem.34.6.843-848.1977 fatcat:ow4bvr6t35fyromxbztzufa7a4