Effect of 10 Weeks of High-Intensity Interval Training on Protein Levels of NF-kB and Expression of Atrogin-1 and MuRF-1 in Cardiomyocytes of Female Mice with Breast Cancer

Naeimeh Yazdanshenas, Maghsoud Peeri, Maryam Delfan, Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran, Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, IranTehran, Iran, Department of Exercise Physiology, Faculty of Sport Sciences, Alzahra University, Tehran, Iran
2020 Iranian Journal of Breast Disease  
Limiting cancer-induced cardiac atrophy is a highly important for improving the survival rates and quality of life in cancer patients. The purpose of this study was to evaluate the effect of 10 weeks of high-intensity interval training (HIIT) on cardiac muscle weight, NF-kB protein expression, and expression of Atrogin-1 and MuRF-1 genes in the heart muscle of breast cancer-bearing female mice. Methods: In this experimental study, 12 BALB/c mice with induced breast cancer were divided into
more » ... e divided into control and HIIT groups. The HIIT protocol consisted of six intervals of running at 85%-90% V O2 peak, interspersed with 90 seconds of active rest, and five minutes of warmup and cool-down. The left ventricular tissue was extracted 24 h following the last training session. Western blotting was used to measure protein levels of NF-kB, and quantitative real-time polymerase chain reaction was used to measure Atrogin-1 and MuRF-1 expression in the heart tissue. Data were analyzed using an independent t test at a 0.05 significance level. Results: The mean cardiac muscle weight in the HIIT group was greater than in the control group (P < 0.001). A significant reduction in NF-kB protein levels (P = 0.004) and expression of Atrogin-1 (P < 0.001) and MuRF-1 (P < 0.001) were observed in the HIIT group when compared with control group. Conclusion: Ten weeks of HIIT may inhibit NF-kB/Atrogin-1/MuRF-1 axis, leading to decreased atrophy and increased heart weight in mice with breast cancer.
doi:10.30699/ijbd.13.3.62 fatcat:p6tdi6w3wnghtb3xugnaot4y3u