Genetic Instability Due to Spindle Anomalies Visualized in Mutants of Dictyostelium

Mary Ecke, Jana Prassler, Günther Gerisch
2021 Cells  
Aberrant centrosome activities in mutants of Dictyostelium discoideum result in anomalies of mitotic spindles that affect the reliability of chromosome segregation. Genetic instabilities caused by these deficiencies are tolerated in multinucleate cells, which can be produced by electric-pulse induced cell fusion as a source for aberrations in the mitotic apparatus of the mutant cells. Dual-color fluorescence labeling of the microtubule system and the chromosomes in live cells revealed the
more » ... ility of spindle arrangements, of centrosome-nuclear interactions, and of chromosome segregation in the atypical mitoses observed.
doi:10.3390/cells10092240 pmid:34571889 pmcid:PMC8469108 fatcat:4phs7xgpo5huvnkpmfkvmybr24