De novo transcriptome assembly of the lobster cockroach Nauphoeta cinerea (Blaberidae)

Ana Lúcia Anversa Segatto, José Francisco Diesel, Elgion Lucio Silva Loreto, João Batista Teixeira da Rocha
2018 Genetics and Molecular Biology  
The use of Drosophila as a scientific model is well established, but the use of cockroaches as experimental organisms has been increasing, mainly in toxicology research. Nauphoeta cinerea is one of the species that has been studied, and among its advantages is its easy laboratory maintenance. However, a limited amount of genetic data about N. cinerea is available, impeding gene identification and expression analyses, genetic manipulation, and a deeper understanding of its functional biology.
more » ... e we describe the N. cinerea fat body and head transcriptome, in order to provide a database of genetic sequences to better understand the metabolic role of these tissues, and describe detoxification and stress response genes. After removing low-quality sequences, we obtained 62,121 transcripts, of which more than 50% had a length of 604 pb. The assembled sequences were annotated according to their genes ontology (GO). We identified 367 genes related to stress and detoxification; among these, the more frequent were p450 genes. The results presented here are the first large-scale sequencing of N. cinerea and will facilitate the genetic understanding of the species' biochemistry processes in future works.
doi:10.1590/1678-4685-gmb-2017-0264 pmid:30043835 fatcat:cmvfc5kxsbhovff4o55uidzimy