Improvement of axial resolution and contrast in temporally focused widefield two-photon microscopy with structured light illumination

Heejin Choi, Elijah Y. S. Yew, Bertan Hallacoglu, Sergio Fantini, Colin J. R. Sheppard, Peter T. C. So
2013 Biomedical Optics Express  
Although temporally focused wide-field two-photon microscopy (TFM) can perform depth resolved wide field imaging, it cannot avoid the image degradation due to scattering of excitation and emission photons when imaging in a turbid medium. Further, its axial resolution is inferior to standard point-scanning two-photon microscopy. We implemented a structured light illumination for TFM and have shown that it can effectively reject the out-of-focus scattered emission photons improving image
more » ... Further, the depth resolution of the improved system is dictated by the spatial frequency of the structure light with the potential of attaining depth resolution better than point-scanning two-photon microscopy.
doi:10.1364/boe.4.000995 pmid:23847726 pmcid:PMC3704103 fatcat:fvdjajourzai7dh47mv4jocxvm