The prevalence and incidence of needlesticks and sharps injuries (NSSIs) have not been well documented in Iran. In most previous studies, the data were obtained through anonymous, self-reporting questionnaires, which are subjected to bias. Objectives: The focus of this study was to investigate the frequency and causes of NSSIs among health care workers (HCWs) in collaboration with an occupational health office and to compare these data with related previous studies. Patients and Methods: In

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... and Methods: In this cross-sectional study, all HCWs exposed to NSSIs in Baharlou Hospital, Tehran, Iran, were included. Among 309 studied cases, 83 HCWs who were directly exposed to NSSIs were enrolled in this study. Results: The case incidence of NSSIs was 26.86% (83.309). Of all the occupational groups, the nurses had the highest rate of NSSI incidence (63.9%, n = 53.83). Moreover, the incidence rate of NSSIs in the HCWs on rotational shift work (90.4%) was higher than that among their counterparts on fixed shift work (9.6%), with the latter chiefly employed on night shift (90.4%, n = 75). NSSIs (83.1%), followed by splash (16.9%), were the most common method of exposure. Conclusions: This study confirmed a relatively high incidence of NSSIs among HCWs. Inadequate occupational health and safety measures such as lack of training on occupational health hazards and safety issues, absence of written protocols for reporting NSSIs, and lack of safety instructions were associated with NSSIs. Effective training programs and raising the awareness of HCWs to reduce unsafe behaviors and implementing organizational strategies to prevent exposure are essential.