O03.4 The vaginal microbiome and development of pelvic inflammatory disease: a prospective study using 16S rRNA long-read sequencing

L Phillips, S Kerry-Barnard, L Zhou, M Furegato, A Witney, P Oakshott, T Sadiq
2021 Bacterial vaginosis   unpublished
by metagenomic sequencing (NovaSeq), and absolute abundance of key bacterial species was assessed by qPCR. Results Immediately following treatment, there was a marked drop in the vaginal level of proinflammatory cytokines (IL-1a, IL-6), chemokines (IL-8, MIP-1b, MIP-3a) and a marker of epithelial barrier disruption (sE-cad; all p<0.01). The vaginal microbiome was generally shifted from BV to community state types (CSTs) dominated by L. iners (49%) or L. jensenii (31%), However, these CST shifts
more » ... were driven by a dramatic (7-12-fold) reduction in the absolute abundance of BV-associated Gram negative anaerobes (Prevotella spp., Gardnerella vaginalis, Atopobium vaginae, and Megasphaera; all p<0.001), while there were minor or no increases in the absolute abundance of L. jensenii (2.9-fold increase, p=0.006) or L. iners (0.6-fold reduction, p=0.245). Conclusions Standard BV therapy reduced inflammatory cytokines/chemokines and enhanced epithelial integrity in the short term. Despite a shift to CSTs dominated by lactobacilli, these immune changes were linked to reductions in the absolute abundance of BV-associated anaerobes rather than to increased Lactobacillus abundance.
doi:10.1136/sextrans-2021-sti.65 fatcat:guvh5vortjejhijb7tzsvov3tq