Exosomes bearing HLA-DR1 molecules need dendritic cells to efficiently stimulate specific T cells

H. Vincent-Schneider
2002 International Immunology  
Exosomes are small vesicles (60±100 nm) secreted by various cell types upon the fusion of endosomal compartments with the plasma membrane. Exosomes from antigen-presenting cells (APC), such as B lymphocytes and dendritic cells (DC), bear MHC class II molecules. In addition, the injection of DC-derived exosomes was reported to elicit potent T cell responses in vivo. Here, we analyzed the activation of speci®c T cells by MHC class II-bearing exosomes in vitro. The rat mast cell line, RBL-2H3, was
more » ... line, RBL-2H3, was engineered to express human class II molecules uniformly loaded with an antigenic peptide [HLA-DR1±hemagglutinin (HA)]. These cells secreted exosomes bearing DR1 class II molecules upon stimulation by a calcium ionophore or IgE receptor cross-linking. Exosomes bearing DR1±HA(306±318) complexes activated HA/DR1-speci®c T cells only weakly, whereas the cross-linking of such exosomes to latex beads increased stimulation of speci®c T cells. By contrast, the incubation of free exosomes with DC resulted in the highly ef®cient stimulation of speci®c T cells. Thus, exosomes bearing MHC class II complexes must be taken up by professional APC for ef®cient T cell activation.
doi:10.1093/intimm/dxf048 pmid:12096030 fatcat:bhl5cfouxfeczhbb3u4nplcwsq