Effects of the root extract of Dipsacus asperoides (Caprifoliaceae) on locomotor function and inflammation following spinal cord injury in rats

Peng Zhang, Faqi Cao, Qirong Dong, Pingyuan Li, Zhaoyao Yang, Xiaoyong Liu, Lei Wang
2017 Tropical Journal of Pharmaceutical Research  
Purpose: The study was performed to determine the effect of Dipsacus asperoides (roots), commonly known as Xu Duan, on rats with spinal cord injury (SCI). Methods: All the animals were separated into 3 groups: normal control; a group that received Dipsacus asperoides treatment after spinal injury, and a group that received phosphate-buffered saline after SCI. These groups allowed for determination of the effect of Dipsacus asperoides treatment on SCI-injured rats. Evaluation of locomotor
more » ... n restoration based on Basso-Beattie-Bresnahan score was carried out while expressions of I-kBα and NF-kB p65 were estimated. Evaluation of pro-apoptotic protein Bax and anti-apoptotic protein Bcl-2 levels was also carried by Western blotting and densitometry. Results: The results showed that 10-mg/kg/day dose of Dipsacus asperoides restored locomotor function in rats after a period of 4 weeks. The treatment also decreased expressions of I-kBα, NF-kB p65 and Bax, but significantly increased expression of Bcl-2 (p < 0.01) in treated rats, compared to untreated rats. Conclusion: The results indicate that treatment with Dipsacus asperoides extract effectively mitigates spinal cord injury by attenuating inflammation and apoptosis in SCI rats.
doi:10.4314/tjpr.v16i6.23 fatcat:rohnghs3czf4deeqrshgvimmci