On-chip microfluidic sorting with fluorescence spectrum detection and multiway separation

Hirokazu Sugino, Kazuto Ozaki, Yoshitaka Shirasaki, Takahiro Arakawa, Shuichi Shoji, Takashi Funatsu
2009 Lab on a Chip  
To examine whether fluorescence spectra were measured with the optical system shown in Fig. 3 , four different fluorescent microspheres were introduced into a multichannel chip. First, Yellow-green microspheres suspended in 10% (w/v) TGP solution were introduced from Inlet 2 using compressed air, while buffer solution containing 10% (w/v) TGP was introduced from Inlet 1. The beads were excited by a blue laser, and the fluorescence spectrum was measured for one minute with this optical system.
more » ... bsequently, fluorescent spectra of the other three microspheres, namely Orange, Red, and Crimson microspheres, were also measured in a similar way. The fluorescence emission spectra of four different microspheres measured by the 1x16 arrayed PMT are shown in Supplemental Fig.1 . The emission maxima of Yellow-green, Orange, Red, and Crimson microspheres were observed at channel Nos. 3, 6, 11, and 13, respectively. By comparing these spectra with those obtained by a fluorescence spectrophotometer (data not shown), we determined that the wavelength range was about 495-685 nm and the bandwidth per channel was about 12 nm. The fluorescence emission spectra of E. coli cells expressing three different fluorescent proteins were also measured by the 1x16 arrayed PMT (Supplemental Fig. 2) .
doi:10.1039/b815765k pmid:19370245 fatcat:wlymdrpvw5c6pnr6vod2ap3uqu