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Adaptive optimisation of illumination beam profiles in fluorescence microscopy
2015
Adaptive Optics and Wavefront Control for Biological Systems
Wide-field fluorescence microscope techniques such as single/selective plane illumination microscope (SPIM) are typically configured to image large regions of a sample at once. Here the illumination beam provides uniform excitation of several biological features across the region, 'sliced' to a thickness of between 5 -10 microns. In this paper we propose a simple alteration to the optical configuration of a SPIM by switching the light-sheetforming cylindrical lens with a spatial light
doi:10.1117/12.2080310
fatcat:kdh3mwspjvarzbpozos2vuob7u