Avian influenza is a threat to global health. This study aims to assess the knowledge, attitudes and practices related to avian influenza among poultry workers in Kathmandu, Nepal.Methods: This is a qualitative cross sectional study done among poultry workers and owners of poultry farms in Kathmandu valley from February 2019 to June 2019 with a face-to-face interview. Data was analysed in the Statistical package of the social sciences (SPSS) version 20.Results: Out of the 100 respondents, 70%

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... re male. 5% were illiterate. 96% of them had knowledge that avian influenza is transferred from uncooked poultry, 57% of them had knowledge regarding symptoms of avian influenza. 57% of them also had knowledge regarding availability of vaccine against the disease with television and newspapers being major sources of information. Among them, 93% applied proper disposal practice regarding poultry wastes, 83% applied masks and boot covers for poultry rearing and 90% maintained hand hygiene before and after coming in contact with poultry.Conclusions: This study highlighted the knowledge among poultry workers about avian influenza, its symptoms and good practices for prevention of its transmission. Television and newspapers appeared to be the most effective means for imparting knowledge regarding avian influenza. Specific age groups should be targeted with appropriate interventions to improve the practices for better prevention of avian influenza.