Integrative Analysis of miRNA and mRNA Expression Profiles in Mammary Glands of Holstein Cows Artificially Infected with Staphylococcus Aureus
Background: Staphylococcus aureus- induced mastitis is one of the most intractable problems for dairy industry, which causes loss of milk yield and early slaughter of cows worldwide. Few study used a comprehensive approach based on the integrative analysis of miRNA and mRNA expression profiles to explore molecular mechanism in bovine mastitis caused by S. aureus. Results: In this study, S. aureus and sterile phosphate buffered saline (PBS) were introduced to different quarters of breasts of
... s of breasts of three individuals, and transcriptome sequencing and microarrays were utilized to detected miRNA and gene expression in mammary gland from the challenged and control groups. A total of 77 differentially expressed microRNAs (DE miRNAs) and 1,625 differentially expressed genes (DEGs) were identified. Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that multiple DEGs were enriched in significant terms and pathways associated with immunity and inflammation. Integrative analysis between DE miRNAs and DEGs proved that miR-664b, miR-23b-3p, miR-331-5p, miR-19b and miR-2431-3p were potential factors regulating the expression levels of CD14 Molecule (CD14), G protein subunit gamma 2 (GNG2), interleukin 17A (IL17A), collagen type IV alpha 1 chain (COL4A1), microtubule associated protein RP/EB family member 2 (MAPRE2), member of RAS oncogene family (RAP1B), LDOC1 regulator of NFKB signaling (LDOC1), low-density lipoprotein receptor (LDLR) and S100 calcium binding protein A9 (S100A9) in bovine mastitis caused by S. aureus. Conclusions: These findings could enhance the understanding of underlying immune response in bovine mammary glands against S. aureus infection, and provide a useful foundation for future application of the miRNA-mRNA based genetic regulatory network in the breeding cows resistant to S. aureus.