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An original bi-enzymatic biosensor was designed by immobilizing Arthrospira platensis cells, called Spirulina, on gold interdigitated transducers. Phosphatase and esterase activities were inhibited, respectively, by heavy metals and by pesticides. Inhibition activities were observed with different mixtures of pesticides + heavy metals. The quantification limits for Cd 2+ and Hg 2+ are 10 −20 M in mixture and in pure solution. The quantification limits of parathion-methyl, paraoxon-methyl anddoi:10.4236/jep.2014.55047 fatcat:zgc62z3uybcypfcnkzfj6mmuru