Is it necessary to soak rice grains to prepare Rhizoctonia solani AG-4 inoculum?

Trazilbo J. Paula Júnior, Rogério F. Vieira, Waldir C. Jesus Junior, Bernhard Hau, Hudson Teixeira
2007 Fitopatologia Brasileira  
A study was conducted to evaluate the effects on the development of root rot on common bean, cv. 'Dufrix' after treatment with four volumes of water (0, 30%, 60%, and 90%, v/w) added to rice grains previously immersed in water for 24 hours before autoclaving and colonization of grains by Rhizoctoniasolani AG-4. Colonized rice grains and non-infested rice grains were mixed in pots with sterilized soil and sand (2:1), where beans were sown. Based on results of area under plant emergence curve,
more » ... nt height, plant dry weight, and disease severity, we conclude that inoculum is more effective in causing disease when no water is added to the rice grains before autoclaving.
doi:10.1590/s0100-41582007000600008 fatcat:7qjvophcs5gspo3lg4mi4u6oo4