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Is it necessary to soak rice grains to prepare Rhizoctonia solani AG-4 inoculum?
A study was conducted to evaluate the effects on the development of root rot on common bean, cv. 'Dufrix' after treatment with four volumes of water (0, 30%, 60%, and 90%, v/w) added to rice grains previously immersed in water for 24 hours before autoclaving and colonization of grains by Rhizoctoniasolani AG-4. Colonized rice grains and non-infested rice grains were mixed in pots with sterilized soil and sand (2:1), where beans were sown. Based on results of area under plant emergence curve,doi:10.1590/s0100-41582007000600008 fatcat:7qjvophcs5gspo3lg4mi4u6oo4