Nitric Oxide Is Important for Mouse Beta-Cell Line Killing by Peritoneal Exudate Cells Obtained from Cyclophosphamide Treated Non-Obese Diabetic Mice

AKIRA KASUGA, TOSHIO NAKAKI, IZUMI TAKEI, SUMIYO TAKAYAMA, YUKAKO OZAWA, TARO MARUYAMA, JUN-ICHI MIYAZAKI, TAKAO SARUTA
1995 Endocrine journal  
Macrophages from recent onset non-obese diabetic (NOD) mice showed cytotoxicity against the NOD mouse derived beta-cell line, MIN6N-9a. In this report, we examined whether nitric oxide is associated with beta-cell destruction. Peritoneal exudate cells (PEC), obtained from cyclophosphamide treated NOD mice showed higher cytotoxicity against MIN6N-9a compared to PECs from saline injected NOD mice (P<0.01). This effect was suppressed in cells incubated with 0.5 mmol/l NG-methyl-L-arginine, a
more » ... L-arginine, a nitric oxide synthase inhibitor (P<0.001). In addition, the nitrite concentration of the co-culture medium, as an index of nitric oxide production, increased in MIN6N-9a cells co-cultured with peritoneal exudate cells from cyclophosphamide injected NOD mice but not in co-culture with saline injected NOD mice (P<0.05). Thus, nitric oxide plays an important role in beta-cell line destruction of macrophages obtained from NOD mice.
doi:10.1507/endocrj.42.259 pmid:7542976 fatcat:q22ga7jh5nf2to74i5lz3owblq