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Evidence for a Mechanism of Recombination during Reverse Transcription Dependent on the Structure of the Acceptor RNA
2003
Journal of Biological Chemistry
Genetic recombination is a major force driving retroviral evolution. In retroviruses, recombination proceeds mostly through copy choice during reverse transcription. Using a reconstituted in vitro system, we have studied the mechanism of strand transfer on a major recombination hot spot we previously identified within the genome of HIV-1. We show that on this model sequence the frequency of copy choice is strongly influenced by the folding of the RNA template, namely by the presence of a stable
doi:10.1074/jbc.m212306200
pmid:12595540
fatcat:25cxpbpg4ffyddzpqv75elbjly