Availability of Nanopore sequences in the genome taxonomy for Vibrionaceae systematics: Rumoiensis clade species as a test case [post]

Mami Tanaka, Sayaka Mino, Yoshitoshi Ogura, Tetsuya Hayashi, Tomoo Sawabe
2018 unpublished
Whole genome sequence comparisons have become essential for establishing a robust scheme in bacterial taxonomy. To generalize this genome-based taxonomy, fast, reliable and cost-effective genomesequencing methodologies are required. MinION, the palm-sized sequencer from Oxford Nanopore Technologies, enables rapid sequencing of bacterial genomes using minim al laboratory resources. Here we tested the ability of Nanopore sequences for the genome-based taxonomy of Vibrionaceae, compared
more » ... compared Nanopore-only assemblies to complete genomes of five Rumoiensis clade species; Vibrio aphrogenes,V. algivorus, V. casei, V. litoralis, and V. rumoiensis. Overall genome relatedness indices (OGRI) based on Nanopore-only assembly were compared with that of Illumina and hybrid assemblies. Our dataset showed that indels in Nanopore-only assemblies does not influence the species discrimination of these five Vibrio species, suggesting the genome sequences from Nanopore-based approach can be used for rapid species identification based on the OGRI.
doi:10.7287/peerj.preprints.26894v1 fatcat:nweoj4o7fjgg7afrllttbm2wdq