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Toluidine blue is a basic thiazine metachromatic dye with high affinity for acidic tissue components, thereby staining tissues rich in DNA and RNA.Toluidine blue has been used in vivo to identify dysplasia and carcinoma of the oral cavity. This article reviews the various vital tissue staining techniques available in the diagnosis of oral precancer and cancer.doi:10.13005/bpj/737 fatcat:5xuek4upczhuzmmvcfpspebhwy