There is evidence that the mammalian ejaculate contains distinct subpopulations of spermatozoa and that the variability among these subpopulations may have adaptive and functional significance. This study investigated the precision, reproducibility and operating characteristics of a novel automated sperm morphology analysis system, the Hobson Morphology package, establishing protocols to investigate boar sperm characteristics. Five ejaculates were collected from each of three boars from

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... t genetic lines: Landrace\p=n-\Meishan introgression, Sireline Large White and Damline Large White. Five semen smears per ejaculate were stained with haematoxylin and eosin. Two hundred spermatozoa per slide were analysed. No significant differences among slides within an ejaculate were detected for sperm tail length (P = 0.770), head width (P = 0.736) and head length (P = 0.615), indicating that both staining and morphology analysis were precise and reproducible. Among the boars, variability in tail length was detected (P = 0.001), but head width (P = 0.114) and length (P = 0.069) did not differ significantly. Multivariate pattern analysis (PATN computer package) highlighted three sub-populations of spermatozoa objectively on the basis of tail length (10.0\p=n-\22.0 \g=m\m, 22.1\p=n-\73.0 \g=m\m and 73.1\p=n-\130.0 \g=m\m). The Landrace\p=n-\Meishan introgression boar possessed more spermatozoa (P < 0.0001) with tails 73.1\p=n-\130 \g=m\m long. Subsequent analysis of morphology parameters in a pure-bred Meishan boar showed similar measurements for tail length (mean \ m=+-\SD; 66.36 \ m=+-\24.70 \g=m\m) to the Landrace\p=n-\Meishan introgression boar (mean \ m=+-\ SD; 67.09 \m=+-\21.80 \g=m\m).Sperm subpopulations originate during spermatogenesis, when heterogeneous genotypic effects determine the structural features of spermatozoa. The findings of this study confirm that tail length differs between boars and that subpopulations of spermatozoa can be detected within a single ejaculate.