Correlation of serum hyaluronic acid with diabetic indices in type 2 diabetes mellitus patients with diabetic nephropathy
International Journal of Clinical Biochemistry and Research
Hyaluronic Acid (HA) is produced by the endothelial smooth muscle cells and adventitial fibroblasts of the arterial wall. In conditions of hyperglycemia, there is an accumulation of HA in blood vessels and can contribute to the diabetic micro-and macrovascular complications. The current study aims to determine the association of serum Hyaluronic Acid levels in Type 2 diabetes mellitus with diabetic nephropathy and its correlation with diabetic indices and renal function test parameters.
... s and Methods: 60 male subjects,30 cases of Type 2 Diabetes Mellitus patients with diabetic nephropathy and 30 non-diabetic healthy subjects as controls. Fasting venous blood samples were collected from cases and controls and analyzed for serum HA by ELISA and HBA1C, FBS, PPBS, urea, and creatinine by Beckmann Coulter Au 480 automated analyser.24 hours urine was collected and analyzed for urinary microalbumin by an immunoturbidimetric method. Statistical analysis was done with SPSS package and p-value < 0.05 was considered significant. Results: Serum HA levels were significantly higher in diabetic patients with nephropathy (75±6 ng/ml) when compared to the normal control groups (25±5 ng/ml). Serum HA also showed a significant positive correlation with FBS, PPBS, HbA1C, urea, creatinine and urine microalbumin. Conclusion: Highly elevated serum Hyaluronic Acid levels and its positive correlation with diabetic indices and renal function test parameters in Type 2 Diabetes Mellitus patients with diabetic nephropathy suggests its association with poor blood glucose control and may possibly predict the development of diabetesrelated vascular complications in Type 2 diabetics without any complications.