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Haplotype-Phased Synthetic Long Reads from Short-Read Sequencing
2016
PLoS ONE
Next-generation DNA sequencing has revolutionized the study of biology. However, the short read lengths of the dominant instruments complicate assembly of complex genomes and haplotype phasing of mixtures of similar sequences. Here we demonstrate a method to reconstruct the sequences of individual nucleic acid molecules up to 11.6 kilobases in length from short (150-bp) reads. We show that our method can construct 99.97%-accurate synthetic reads from bacterial, plant, and animal genomic
doi:10.1371/journal.pone.0147229
pmid:26789840
pmcid:PMC4720449
fatcat:k3dhbedgdbawtoowdhdkvrzq5i