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Determination of ploidy and proliferative characteristics of human solid tumors by pulse cytophotometry
1978
Cancer Research
Cellular DNA content is a discriminator of cell cycle stage and ploidy. Using pepsin digestion for cell disper sal, a combination of ethidium bromide and mithramycin for DNA fluorochromation, and a new sheath flow cham ber in a PHYWE ICP-11 pulse cytophotometer, we ob tained DNA histograms with high resolution (coefficient of variation, 2.0 to 7.0; mean, 3.3%) on biopsy samples of solid tumors. There were 78 observations in 26 patients (malignant melanoma, 7; breast carcinoma, 4; lung carci
pmid:688223
fatcat:xjiaaknywbc7difkyvwxegoahy