Phosphofructokinase determination

M. Kapoor
1969 Fungal Genetics Reports  
Phosphofructokinase determination Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This enzyme methodology is available in Fungal Genetics Reports: Kopeor, M. Phosphofructokinase Neurmparo crosso fluffyoid strain P62B (FGSCt553) was used as o source of of Neurosporo crosso. All cultures were prepared in Vcgel's minimal medium with 2% sucrose as the carbon source.
more » ... e carbon source. Conidiol Suspensions containing co. 5 x lo7 conidia/ml were prepared from agar slants grown for 6 doys at 28X. Liquid cultures were started by inoculating 5 ml of the conidiol suspension into a 2.8 I Fernbach flask containing 1.5 I of the medium and grown in on environmental rotatory shaker (New Brunswick ) at 2B'C for 30 h n; the mycelia were then harvested, lyophilized and stored at -2O'C. Assay methods: PFK was assayed by two methods. Method 1, a slight modification of that of Uyeda and Racker (1965 J. Biol. Chem. 240:4682), consisted in using a reaction system containing the follaving: Tris-HCI, pH 8.0, 100 pm&s ATP I. 25 pmoles;
doi:10.4148/1941-4765.2057 fatcat:jhbsb6pfzzftnf6koc6udbucca