cDNA clone of hepatitis A virus encoding a virulent virus: induction of viral hepatitis by direct nucleic acid transfection of marmosets

S U Emerson, M Lewis, S Govindarajan, M Shapiro, T Moskal, R H Purcell
1992 Journal of Virology  
Direct inoculation of marmoset livers with an in vitro transcription mixture containing cDNA and full-length genomic RNA transcripts of hepatitis A virus resulted in acute viral hepatitis. Elevations in serum levels of liver enzymes were correlated with appearance of antibody to hepatitis A virus. Genomes of infectious hepatitis A virus isolated from the feces of transfected marmosets contained the same mutation as the cDNA template used for transfection. Liver biopsies confirmed that the virus
more » ... encoded by the cDNA clone induced histopathological changes equivalent to those caused by virulent wild-type virus.
doi:10.1128/jvi.66.11.6649-6654.1992 fatcat:unfps6u7hzdepi33m2aqjnuzbi