Antibody preparation and identification of the Cashmere goat c-kit protein in the testes

S.C.L. Wu, F.H. Luo, Q.F. Kong, Y.J. Wu
2014 Genetics and Molecular Research  
The c-kit protein plays a major role in the regulation of germ cell development. Its expression and distribution in rodent testes have been widely reported. However, research regarding c-kit expression in domestic animals is scarce, and the expression pattern and distribution of c-kit in germ cells have not been clearly defined. In this study, a specific antigenic region for goat c-kit was designed, and a c-kit polyclonal antibody was prepared. This antibody was then applied in a study
more » ... in a study evaluating c-kit expression in Cashmere goat tissues. A Western blot analysis showed that three forms of c-kit were expressed in goat testes: precursor, mature, and soluble c-kit. Fluorescent immunohistochemical analyses showed that c-kit was primarily expressed in the spermatogonia and spermatocytes of goat testes. These results not only clarify the expression and localization of c-kit in the 4857 ©FUNPEC-RP Genetics and Molecular Research 13 (3) : 4856-4866 (2014) c-kit in Cashmere goat testes goat testis, but also accelerate further research regarding the function of c-kit in goat spermatogenesis.
doi:10.4238/2014.july.2.13 pmid:25062419 fatcat:vvq4lolq6vhyxhtmg2n3deoqcq