Promotion of platelet aggregation by sera from brucellosis patients with antiphosphatidylcholine antibodies

M. ÁNGELES CASAO, RAMÓN DÍAZ, ANTONIO ORDUÑA, CARLOS GAMAZO
2001 Journal of Medical Microbiology  
Results obtained in this study suggest that in human brucellosis there is an antibody response against platelet-activating factor (PAF) and phosphatidylcholine (PC). The speci®city of the antiphospholipid response was determined by inhibition assays. The PAF molecule was able to inhibit the anti-PC activity of the brucellosis-control serum. This inhibition capacity of PAF was similar to that of the phosphorylcholine (PYC) group. These results suggest that the inhibition activity could be
more » ... ity could be attributed to the PYC group present in both PAF and PC molecules. Consequently, these ®ndings support an immunodominant role of PYC in the antiphospholipid response of brucellosis. Furthermore, sera from patients infected with Brucella organisms were able to cause platelet aggregation, as were brucella phospholipids, suggesting a possible role of the antiphospholipid antibodies and phospholipids in the in¯ammatory response in brucellosis.
doi:10.1099/0022-1317-50-11-965 pmid:11699593 fatcat:nmrphhzm6fh7rhwxxejp7yviam