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Cryoelectron-Microscopy Image Reconstruction of Symmetry Mismatches in Bacteriophage φ29
2001
Journal of Structural Biology
A method has been developed for three-dimensional image reconstruction of symmetry-mismatched components in tailed phages. Although the method described here addresses the specific case where differing symmetry axes are coincident, the method is more generally applicable, for instance, to the reconstruction of images of viral particles that deviate from icosahedral symmetry. Particles are initially oriented according to their dominant symmetry, thus reducing the search space for determining the
doi:10.1006/jsbi.2001.4379
pmid:11562164
pmcid:PMC5595366
fatcat:4kluevoklfa6npcuvp6n5brskq