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Etienne Van den Abbeel 1 and growing ice crystals at the advancing ice front (Leibo et al., 1978; Rall et al., 1984; Ashwood-Smith et al., 1988). When André Van Steirteghem cryopreserved mammalian embryos are recovered from liquid Centre for Reproductive Medicine, University Hospital and Medical nitrogen, therefore, embryos with damaged blastomeres and/ School, Dutch-speaking Brussels Free University (Vrije Universiteit or with a cracked zona pellucida (ZP) are found (Lehn-Jensen Brussel), Brussels, Belgium and Rall , 1983; Rall et al., 1984) .doi:10.1093/humrep/15.2.373 pmid:10655309 fatcat:nxioxlzqdzex7d2mxhvlrhvrv4