Enhancement of Paclitaxel Production in Plant Cell Culture Including Increased Amount of Water-immiscible 1-Butyl-1-methylpyrrolidinium Bis(trifluoromethanesulfonyl)imide

Shinjiro YAMAMOTO, Yumi KAMIKUBO, Hiroki TANOUE, Michinari IWASAKI, Shuhei HAYASHI, Hitoshi MIYASAKA
2021 Solvent extraction research and development, Japan  
We report here the effect of increased amount of a water-immiscible ionic liquid (IL), 1-butyl-1-methylpyrrolidinium bis(trifluoromethanesulfonyl)imide (P14-TFSI), enhancing the production amount of paclitaxel with in situ extraction from an aqueous medium in a plant cell culture. The productivity of paclitaxel in the culture including the IL of 10 vol% was 1.5 times greater than that of 2.5 vol%. Moreover, addition of an elicitor, methyl jasmonate (MJ) enhanced the production amount of
more » ... el in the culture including 10% P14-TFSI. The amount of paclitaxel in the culture including 1 µM MJ and 10 vol% P14-TFSI was more than 78 times greater than those in the control culture without both P14-TFSI and MJ, and two times greater than those in the culture including 2.5 vol% P14-TFSI alone.
doi:10.15261/serdj.28.109 fatcat:ruiybdvupveu7b62f23avdra3u