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Genetic and phosphoproteomic basis of LysM-mediated immune signaling in Marchantia polymorpha highlights conserved elements and new aspect of pattern-triggered immunity in land plants [article]

Izumi Yotsui, Hidenori Matsui, Shingo Miyauchi, Hidekazu Iwakawa, Katharina Melkonian, Titus Schluter, Selena Gimenez Ibanez, Takehiko Kanazawa, Yuko Nomura, Sara Christina Stolze, Hyung-Woo Jeon, Shigeo S Sugano (+7 others)
2022 bioRxiv   pre-print

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https://web.archive.org/web/20230103150638/https://www.biorxiv.org/content/biorxiv/early/2022/12/30/2022.12.28.521631.full.pdf

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Pattern-recognition receptor (PRR)-triggered immunity (PTI) wards off a wide range of pathogenic microbes, playing a pivotal role in plant immunity. The model liverwort Marchantia polymorpha is emerging as a popular model for investigating the evolution of plant-microbe interactions. M. polymorpha triggers defense-related gene expression upon sensing components of bacterial and fungal extracts, suggesting the existence of PTI in this plant model. However, the molecular components of the
more » ... PTI in M. polymorpha have not yet been described. We show that, in M. polymorpha, which has four LysM receptor homologs, lysin motif (LysM) receptor-like kinase (LYK) MpLYK1 and LYK-related (LYR) MpLYR are required for sensing chitin and peptidoglycan fragments, triggering a series of characteristic immune responses. Comprehensive phosphoproteomic analysis of M. polymorpha in response to chitin treatment identified regulatory proteins that potentially shape LysM-mediated PTI. The identified proteins included homologs of well-described PTI components in angiosperms as well as proteins whose roles in PTI are not yet determined, including the blue-light receptor phototropin MpPHOT. We revealed that MpPHOT is required for a negative feedback of defense-related gene expression during PTI. Taken together, this study outlines the basic framework of LysM-mediated PTI in M. polymorpha and demonstrates the utility of M. polymorpha as a plant model for discovering novel or fundamental molecular mechanisms underlying PRR-triggered immune signaling in plants.
doi:10.1101/2022.12.28.521631 fatcat:eq2kytco6rcpdf5anlrfyr7hby
Citation

Izumi Yotsui, Hidenori Matsui, Shingo Miyauchi, Hidekazu Iwakawa, Katharina Melkonian, Titus Schluter, Selena Gimenez Ibanez, Takehiko Kanazawa, Yuko Nomura, Sara Christina Stolze, Hyung-Woo Jeon, Shigeo S Sugano, Makoto Shirakawa, Ryuichi Nishihama, Yasunori Ichihashi, Ken Shirasu, Takashi Ueda, Takayuki Kohchi, Hirofumi Nakagami. "Genetic and phosphoproteomic basis of LysM-mediated immune signaling in Marchantia polymorpha highlights conserved elements and new aspect of pattern-triggered immunity in land plants." bioRxiv (2022)