A development of a live-cell observation method and analyses of the effect of blood molecules on the cell adhesion on the surface of Polytetrafluoroethylene 2016 年 9 月 麻布大学大学院 獣医学研究科 動物応用科学専攻 動物工学 DA1361 犬飼 直人 図表 39 i Abstract Polytetrafluoroethylene (PTFE), a kind of fluoropolymer, is widely used as a biomaterial for blood-contacting medical devices because of its properties, such as good biocompatibility, chemical stability and anti-cellular adhesiveness. In blood vessel prostheses, the

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... latelets adhesiveness and endothelialization on the surface of PTFE have been studied for the suppression of thrombus formation. Especially, the endothelialization plays an important role for the longstanding suppression of thrombus formation, the stability of the endothelial adhesiveness on the PTFE is important for that. Inverted phase-contrast microscopy is suitable for the chronological observation, which is needed to estimate the stability of the cellular adhesiveness on the PTFE. However, it is difficult to observe on the surface of thick PTFE, which have sufficient mechanical strength for use in biological experiments, by inverted phase-contrast microscopy because of its low optical transmittance. Although a thin layer of PTFE is likely to have low optical transmittance sufficient for observation under an inverted phase-contrast microscope such a thin layer would have insufficient mechanical strength. In the present study, we developed a PTFE-coated glass with optical transmittance sufficient for observation under an inverted phase-contrast microscope but with sufficient mechanical strength for use in biological experiments. The purpose of this thesis is that the observation of cells under an inverted phase-contrast microscope and the analysis of the effect of blood molecules on the cell adhesion on the surface of PTFE by using the PTFE-coated glass. The background of this thesis was explained in the first chapter. The PTFE-coated glass, which was made by the PTFE coating on the cover glass, was developed to achieve these opposite properties, the optical transmittance and ii mechanical strength, in the second chapter. Rat aortic endothelial cells (RAOECs) were seeded on the PTFE-coated glass treated with plasma that achieves the cell adhesion to PTFE. The observation of RAOECs on the PTFE-coated glass was tried by using an inverted phase-contras microscope and an inverted fluorescence microscope. It was possible to observe RAOECs on the surface of the PTFE-coated glass by using these microscopes. Any recognizable damage or peeling was recognized in the present study.