Thesis submitted to the University of Sri Jayawardenepura for the award of the degree of Master of Philosophy in Chemistry. July 2000 Pa e LIST OF CONTENTS (i) LIST OF TABLES (ix) LIST OF FIGURES (xi) 1. 1.9.3 Abuse of Datura 46 1.9.4 Pharmacological effect 48 1.9.5 Identification 48 1.10 Objectives of the present study 48 2. EXPERIMENTAL 52 2.1 Equipment and materials 2.1.1 Equipment 2.1.2 Materials 2.2 Samples 2.2.1 Standard samples 2.2.2 Madana Modakaya (MM) 2.2.3 Cannabis plants 2.3 Total

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... traction of A 9-THC 54 2.3.1. Determination of the best solvent for 54 extraction of t9 -THC from Cannabis leaves and MM 2.3.1.1 Cannabis leaves 54 2.3.1.2 MM 55 2.4 Qualitative analysis 55 2.4.1 Thin Layer Chromatography (TLC) 55 2.4.1.1 Developing solvent system 55 2.4.1.2 Visualization 56 2.4.1.2.1 Preparation of visualization 56 reagent 2.4.2 Gas Chromatography (CC) 56 2.4.2.1 Gas Chromatographic apparatus 2.4.2.2 Determination of optimum oven temperature 2.5 Quantitative analysis of A9-THC in MM samples 2.5.1 Extraction 2.5.2 Column Chromatography 2.5.3 Standard curve 2.5.4 Determination of accuracy of the method 2.6 Determination of A9-Tetrahydrocannahinolic acid 61 ( 9-THCA) and 9-THC content in leaf and MN 2.6.1 Leaf samples 61 2.6.2 MM samples 2.7 Extractability of 9-THC in ghee, coconut oil and sesame oil at 140 2.7.1 Extraction 2.7.2 Densitometric determination of A9-THC content 2.7.