Quantification of Flavonoids and Nucleoside by UPLC-MS in Indian Cordyceps sinensis and its invitro Cultures

Masood Shah Khan, Washim Khan, Manimaran Manickam, Raj kumar Tulsawani, Kshipra shipra Misra, Puran Singh Negi, Sayeed Ahmad
2015 Indian Journal of Pharmaceutical Education and Research  
Cordyceps sinensis, a comestible mushroom growing in Himalayan regions, is extensively familar in traditional system of medicine. The paper presents the results of quantification of some of the flavonoids and nucleosides (kaempferol, isorhamnetin and cordycepin) in the Indian variety of Cordyceps sinensis. Since, the plant is known for its high therapeutic potential and not easily available, hence it was also tissue cultured in the laboratory as an alternative source of medicine. Methods: Ultra
more » ... Performance Liquid Chromatography (UPLC) along with Q-TOF-MS detector was used for the characterization and quantification of active components in the aqueous extracts of both the samples of Cordyceps sinensis (natural as well as in vitro cultured). Results: Results indicate that both the extracts have almost similar concentration of the kaempferol, isorhamnetin and cordycepin which supports use of in vitro biomass as an alternative source of medicine.
doi:10.5530/ijper.49.4s.7 fatcat:f7w65hyfgzf5fjfx4kr3afx7la