Despite intensive research of DNA repair after UV in eukaryotes, a framework to quantitatively describe the dynamics in vivo is still lacking. We developed a new data-driven approach to analyse CPD repair kinetics over time in Saccharomyces cerevisiae. In contrast to other studies that consider sequencing signals as an average behaviour, we introduce a hidden axis representing independent cells where loci can transition from damaged to repaired. This permits the application of the

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... nson-Mehl-Avrami model to find a region-specific and continuous representation of the entire temporal process. We correlated the parameters via a k-nearest neighbour approach to a variety of genomic features, including transcription rate and nucleosome density. The clearest link was found for the gene size, which has been unreported for budding yeast to our knowledge. The framework hence allows a comprehensive analysis of nuclear processes on a population scale.