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Tissue-specific tagging of endogenous loci inDrosophila melanogaster
Fluorescent protein tags have revolutionized cell and developmental biology, and in combination with binary expression systems they enable diverse tissue-specific studies of protein function. However these binary expression systems often do not recapitulate endogenous protein expression levels, localization, binding partners, and developmental windows of gene expression. To address these limitations, we have developed a method called T-STEP (Tissue-SpecificTagging ofEndogenousProteins) thatdoi:10.1101/025635 fatcat:fkrv7evpovcrlgtvgatbr5wj3a