Immunogenicity in dogs of three recombinant antigens (TSA, LeIF and LmSTI1) potential vaccine candidates for canine visceral leishmaniasis

Ricardo Toshio Fujiwara, André Macedo Vale, João Carlos França da Silva, Roberto Teodoro da Costa, Josiane da Silva Quetz, Olindo Assis Martins Filho, Alexandre Barbosa Reis, Rodrigo Corrêa Oliveira, George Lins Machado-Coelho, Lilian Lacerda Bueno, Jeffrey Michael Bethony, Glen Frank (+5 others)
2005 Veterinary Research  
Control of canine visceral leishmaniasis (VL) remains a difficult and serious problem mostly because there is no reliable and effective vaccine available to prevent this disease. A mixture of three recombinant leishmanial antigens (TSA, LeIF and LmSTI1) encoded by three genes highly conserved in the Leishmania genus have been shown to induce excellent protection against infection in both murine and simian models of cutaneous leishmaniasis. A human clinical trial with these antigens is currently
more » ... tigens is currently underway. Because of the high degree of conservation, these antigens might be useful vaccine candidates for VL as well. In the present study, using the dog model of the visceral disease, we evaluated the immunogenicity of these three antigens formulated with two different adjuvants, MPL-SE ® and AdjuPrime ® . The results were compared with a whole parasite vaccine formulated with BCG as the adjuvant. In order to investigate if sensitization with the recombinant antigens would result in recognition of the corresponding native parasite antigens upon infection, the animals were exposed for four weeks after the termination of the immunization protocol with the recombinant antigens to a low number of L. chagasi promastigotes, an etiological agent of VL. Immune response was evaluated by quantitative ELISA in the animal sera before and after exposure
doi:10.1051/vetres:2005033 pmid:16120256 fatcat:2cyntrf7krfkxi3r4ilg6wucx4