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Horticultural Research (Japan)
In order to develop an efficient procedure for chromosome doubling of lavandin, leaf-derived calli were treated with colchicine. Relatively high adventitious shoot formation rates, from 71.8 to 79.3%, were obtained in the medium with 0.05, 0.1 and 0.5% of colchicine, although almost all shoots showed hyperhydricity. However, root formation rates were seriously decreased. Conclusively, 2, 16 and 7 chromosome-doubled plants were obtained from calli treated with 0.05, 0.1 and 0.5% of colchicine,doi:10.2503/hrj.10.303 fatcat:yd5knissajdgfoqdxcvbhu67n4