The role of soil pH on soil carbonic anhydrase activity

Joana Sauze, Samuel P. Jones, Lisa Wingate, Steven Wohl, Jérôme Ogée
2017 Biogeosciences Discussions  
Carbonic anhydrases (CAs) are metalloenzymes present in plants and microorganisms that catalyse the interconversion of CO<sub>2</sub> and water to bicarbonate and protons. Because oxygen isotopes are also exchanged during this reaction, the presence of CA also modifies the contribution of soil and plant CO<sup>18</sup>O fluxes to the global budget of atmospheric CO<sup>18</sup>O. The oxygen isotope signatures (<i>&amp;delta;</i><sup>18</sup>O) of these fluxes differ as leaf water pools are
more » ... ater pools are usually more enriched than soil water pools, and this difference is used to partition the net CO<sub>2</sub> flux over land into soil respiration and plant photosynthesis. Nonetheless, the use of atmospheric CO<sup>18</sup>O as a tracer of land surface CO<sub>2</sub> fluxes requires a good knowledge of soil CA activity. Previous studies have shown that significant differences in soil CA activity are found in different biomes and seasons but our understanding of the environmental and ecological drivers responsible for the spatial and temporal patterns observed in soil CA activity is still limited. One factor that has been overlooked so far is pH. Soil pH is known to strongly influence microbial community composition, richness and diversity in addition to governing the speciation of CO<sub>2</sub> between the different carbonate forms. In this study we investigated the CO<sub>2</sub>&amp;ndash;H<sub>2</sub>O isotopic exchange rate (<i>k</i><sub>iso</sub>) in six soils with pH varying from 4.5 to 8.5. We also artificially increased the soil CA concentration to test how pH and other soil properties (texture and phosphate content) affected the relationship between <i>k</i><sub>iso</sub> and CA concentration. We found that soil pH was the primary driver of <i>k</i><sub>iso</sub> after CA addition and that the chemical composition (i.e. phosphate content) played only a secondary role. We also found an offset between the <i>&amp;delta;</i><sup>18</sup>O of the water pool with which CO<sub>2</sub> equilibrates and total soil water (i.e. water extracted by vacuum distillation) that varied with soil texture. The reasons for this offset are still unknown.
doi:10.5194/bg-2017-214 fatcat:hnvjic2zxrc4xndkcbw6i6y3pm