Recebido em 8/1/10; aceito em 1/9/10; publicado na web em 7/1/11 DEVELOPMENT OF ANALYTICAL METHODS FOR HPLC-UV TO THE ANTIMICROBIANS TETRACYCLINE, SULFA-METHOXAZOLE AND TRIMETHOPRIM USING ZIRCONOCENE-BASED SILICA AS PRE-CONCENTRATION/ EXTRACTION SYSTEMS. This paper evaluates the adsorption capacity of zirconocene-based silica materials in the preconcentration of antimicrobians (tetracycline, sulfamethoxazole and trimethoprim) in aqueous medium. These materials were prepared by grafting the

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... nocene onto silicas pre-treated at different temperatures. The retention capacity of these materials was evaluated by off line SPE and HPLC-UV and the proposed methodology was validated in ultrapure, tap and river water. The recovery for tetracycline was 72% (in the solid phase A) and, for sulfamethoxazole and trimethoprim was 68 and 95% in the commercial C18, respectively. The target antimicrobians were not detected in the Arroio Dilúvio (Porto Alegre -RS).