Quantitative Phase Velocimetry for Label-Free Measurement of Intracellular Mass Transport Velocity [article]

Soorya Pradeep, Thomas A Zangle
2021 bioRxiv   pre-print
Transport of mass within cells helps maintain homeostasis and is disrupted by disease and stress. Here, we develop quantitative phase velocimetry (QPV) as a label-free approach to make the invisible flow of mass within cells visible and quantifiable. We benchmark our approach against alternative image registration methods, a theoretical error model, and synthetic data. Our method tracks not just individual labeled particles or molecules, but the entire flow of material through the cell. This
more » ... bles us to measure diffusivity within distinct cell compartments using a single approach, which we use here for direct comparison of nuclear and cytoplasmic diffusivity. As a label-free method, QPV can be used for long-term tracking to capture dynamics through the cell cycle. Finally, based on the known effective particle size, we show that QPV is an accessible method to measure intracellular viscosity.
doi:10.1101/2021.08.04.452408 fatcat:wnepgqobkbhuhk6ba3i5z2qk44