Cholinesterases Inhibition by Novelcis- andtrans-3-ArylaminocyclohexylN,N-Dimethylcarbamates: Biological Evaluation and Molecular Modeling

Diego A. S. Yamazaki, Augusto A. Cândido, Mariane C. Bagatin, Miguel Machinski Jr., Simone A. G. Mossini, Rodrigo M. Pontes, Fernanda A. Rosa, Ernani A. Basso, Gisele F. Gauze
2016 Journal of the Brazilian Chemical Society  
The present study describes the synthesis, assessment of the anticholinesterase activity and the inhibition type of novel cis-and trans-3-arylaminocyclohexyl N,N-dimethylcarbamates. In vitro inhibition assay by Ellman's method with human blood samples showed that carbamates were selective for butyrylcholinesterase (BuChE) with compound concentration that inhibits 50% of enzyme activity (IC 50 ) between 0.11 and 0.18 mmol L -1 . cis-and trans-3-(4-Methoxyphenylamino) cyclohexyl
more » ... l N,N-dimethylcarbamate hydrochloride were the most active for BuChE, showing that the presence of methoxyl group enhanced the anticholinesterase activity. The enzyme kinetics studies indicate a noncompetitive inhibition against acetylcholinesterase (AChE) and mixed type inhibition for BuChE. Molecular modeling studies confirm the ability of carbamates to bind both the active and peripheral sites of the BuChE.
doi:10.5935/0103-5053.20160041 fatcat:k45s6sipvjczpplifx4vh6cxqq