Cholinesterases Inhibition by Novelcis- andtrans-3-ArylaminocyclohexylN,N-Dimethylcarbamates: Biological Evaluation and Molecular Modeling
Journal of the Brazilian Chemical Society
The present study describes the synthesis, assessment of the anticholinesterase activity and the inhibition type of novel cis-and trans-3-arylaminocyclohexyl N,N-dimethylcarbamates. In vitro inhibition assay by Ellman's method with human blood samples showed that carbamates were selective for butyrylcholinesterase (BuChE) with compound concentration that inhibits 50% of enzyme activity (IC 50 ) between 0.11 and 0.18 mmol L -1 . cis-and trans-3-(4-Methoxyphenylamino) cyclohexyl
... l N,N-dimethylcarbamate hydrochloride were the most active for BuChE, showing that the presence of methoxyl group enhanced the anticholinesterase activity. The enzyme kinetics studies indicate a noncompetitive inhibition against acetylcholinesterase (AChE) and mixed type inhibition for BuChE. Molecular modeling studies confirm the ability of carbamates to bind both the active and peripheral sites of the BuChE.