Optimum Condition of High Pressure Treatment for the Preparation of Lysozyme-Dextran Complex Found by Random-Centroid Optimization

Kenjin NAKAMURA, Satoshi HATANO, Akiyoshi MATSUOKA, Noboru FURUKAWA, Tsuyoshi TAKAHASHI, Yoshitada YAMANAKA
2001 Food science and technology research  
The lysozyme-dextran complex (LDC) was prepared in the liquid state using high pressure treatment. This method was able to shorten the reaction period for the preparation of LDC and to suppress the formation of melanoidin, although the production rate of LDC was decreased. The maximum production rate of LDC was attained by 20 experiments using the random-centroid optimization (RCO) method. The optimum condition for the preparation of LDC was pH 4.5, 0.1 M NaCl, 192 MPa, 19.3˚C and the treatment
more » ... C and the treatment time was 88 min, under which one-tenth of the lysozyme formed the complex. Optimization by the RCO method was successful in this study, and is reasonably expected to be a practical and powerful tool in various research fields. . Optimum composition of ingredients for high-fiber bread containing wheat bran by random centroid optimization. Bull. Fuji Women's Coll., 35. . Novel bifunctional lysozyme-dextran conjugate that acts on both gram-negative and gram-positive bacteria. . Optimum preparative method for storing cream puff paste without deterioration. Food Sci. Technol. Int. Tokyo., 4, 18-24. Perez-Mateos, M. and Montero, P. (1997). High-pressure-induced gel of sardine (Sardina pilchardus) washed mince as affected by pressure-time-temperarure. . A global optimization algorithm (GOP) for certain class of non convex NLPsII. Appication of theory and test problems. Comput. Chem. Eng., 14, 1419-1434. Yassen, H.A. (1993. A level set global optimization method for nonlinear engineering problems. Ph.D. Thesis. University of British Columbia. Fig. 3. Graphical results of the maximization of the production rate of lysozyme-dextran complex prepared in the liquid state using high pressure treatment.
doi:10.3136/fstr.7.84 fatcat:4mtzhqdnx5aodb6iwtkkvy7bfa